Biowing applied biotechnology
WebJun 24, 2015 · All genotyping experiments were performed by Shanghai BioWing Applied Biotechnology (www.biowing.com.cn). Genomic DNA was isolated from peripheral blood using an AxyPrep-96 (AXYGEN) kit, and target DNA sequences amplified using a multiplex polymerase chain reaction (PCR) method. After PCR, genotyping was carried out using … WebJul 7, 2024 · Human CRC cell lines (SW480 and HCT116), requested from the “Chinese Academy of Sciences”, were confirmed fingerprinting using “STR DNA” by Shanghai Biowing Applied Biotechnology Co., Ltd. (Shanghai, China). A Mycoplasma Stain Assay Kit (Beyotime, C0296) was used to identify the mycoplasma infection.
Biowing applied biotechnology
Did you know?
WebDec 15, 2024 · Blood samples were collected from all participants using K2EDTA tubes and a Blood Genotyping DNA Extraction Kit. The genotype of the SNP was analyzed by the Shanghai Biowing Applied Biotechnology Co. Ltd. (www.biowing.com.cn) using the Ligase Detection Reaction-Polymerase Chain Reaction method. Genomic DNA extracted … WebMar 2, 2024 · by Shanghai Biowing Applied Biotechnology CO. LTD, as described previously (15). qPCR was conducted in triplicate, and the reactions included 4 l of …
WebNov 14, 2024 · All cell lines in this study were authenticated using STR DNA fingerprinting, most recently in March 2024 by Shanghai Biowing Applied Biotechnology Co., Ltd (Shanghai, China), and mycoplasma infection was detected using LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich). Only lower passage cell lines were used for the study. WebSep 13, 2024 · The identity of the cell lines was verified by short tandem repeat (STR) profiling (Shanghai Biowing Applied Biotechnology), absence of Mycoplasma infection was verified by conventional PCR with the PromoKine PCR Mycoplasma Test Kit I/C. All stable cell lines were aliquoted and frozen after derivation and expansion, experiments …
WebDec 16, 2024 · profiling by Shanghai Biowing Applied Biotechnology Co, Ltd on February 28, 2024. The parental K562 cells were cultured in RPMI medium (Cromwell) with 10% fetal bovine serum (Waltham) and 1% penicillin–streptomycin (HyClone) at 37 C with 5% CO 2 and 95% humidity. The building of drug-resistant K562 cell models includes … WebMay 1, 2024 · Twenty-one loci were verified by Biowing Applied Biotechnology (Shanghai, China) as previously described (Wang et al., 2024). Declaration of competing …
http://www.biowing.com.cn/p-about.html
WebTesting Company: Shanghai Biowing Applied Biotechnology Co. Ltd Address: Room 502, NO.1015 Longteng Rd , Songjiang District, Shanghai Tel: +86-021-33559491 Contact: Wenyao Zhang E-mail: [email protected] . SHANGHAI BIOWING BIOTECHNOLOGY Co. LTD Your Intimate Partner in Bioscience! imc chelmsfordWebMar 14, 2024 · Before experiments were performed, short tandem repeat assays were used to authenticate all cell lines (Shanghai Biowing Applied Biotechnology Co., Ltd.). Stable transfection. Human SLC38A2 short hairpin (sh)-RNA and empty vector lentiviral particles were constructed by Qingke Co., Ltd. The sh1-SLC38A2 target sequence was 5 ... imcc healthWebDec 16, 2024 · The Hep-G2 cells (Shanghai Biowing Applied Biotechnology Co., Ltd.) were authenticated as a hepatocellular carcinoma cell line using short tandem repeat profiling, which was also conducted by Shanghai Biowing Applied Biotechnology Co., Ltd. The cells were maintained in RPMI-1640 (Gibco) supplemented with 10% (v/v) fetal … list of justinian code lawsWebmethod in Shanghai Biowing Applied Biotechnology Co. Ltd. (Shanghai, China) on July 4, 2024, and July 11, 2024. PCR was used to detect whether the cells were contaminated with mycoplasma once a wk. All the experiments were com - pleted within 6 mo after receiving cells from the cell bank. U251 and A172 cells were grown in Dulbecco’s modied list of just william booksWebFeb 5, 2008 · Hypericin, extracted from H. perforatum, can induce the generation of reactive oxygen species by visible light irradiation, which may consequently induce the conformational change of hemoglobin. We have not only employed UV-vis spectroscopy to observe the changes of UV-vis spectra of the protein, which reveals the conformational … list of k1 companiesWebDeadline 1: January 31, 11:59pm Pacific Time: Priority application deadline. Applications received prior to January 31st, will be reviewed in mid-late February with committee … imc cherry pointWebMar 31, 2016 · View Full Report Card. Fawn Creek Township is located in Kansas with a population of 1,618. Fawn Creek Township is in Montgomery County. Living in Fawn … imc chipper parts