Cisplatin assay
WebSep 30, 2024 · Cisplatin (cis-diamminedichloridoplatinum (II), CDDP) is one of the most commonly used antineoplastic drugs worldwide. The platinum-based compound has been in clinical use for more than 40 years and is a cost-effective first-in-line treatment against several solid cancers including ovarian, head and neck and testicular cancer ( 1 ). WebMar 1, 2024 · The developed and validated assay presented here is a highly sensitive LC-MS/MS method developed and validated for the quantitative determination of cisplatin following derivatization with diethyldithiocarbamate (DDTC) in order to detect platinum (Pt) of cisplatin, suitable for pharmacokinetic studies in rats and to further use it to study …
Cisplatin assay
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WebNov 1, 2005 · The assay was calibrated by addition of known amounts of cisplatin to drug-free plasma as described previously. Samples used for calibration contained cisplatin … WebBased on my testing, the best way is to dissolve cisplatin in 0.9% NaCl, and this approach can keep cisplatin stable not only at room temperature but also at 37-41°C. The solubility of...
WebAug 13, 2024 · Annexin V/propidium iodide (PI) apoptosis assay Cells were seeded onto 6-well plates at a density of 2.5 × 10 5 cells/well and assigned into four groups. In the single treatment group, cells were... http://www.pharmacopeia.cn/v29240/usp29nf24s0_m17910.html
After observing the enhanced cell-killing efficacy of the combination of CDDP and BV10 in various cancer cell lines and studying cell death processes, we further investigated the anti-cancer effect of this combination in the xenograft mouse models of lung, ovarian and cervical cancers. The typical dose of … See more The results of the effect of the combination of CDDP with BV10 on in vitro cell viability and clonogenic death are shown in Fig. 3. Figure 3A shows the … See more To demonstrate the DET mechanism of the combination, we made real-time measurements of the reaction between CDDP and BV10 … See more Figure 4A shows representative images of HeLa cells treated by CDDP with/without the combination with BV10 for 12 h, where apoptotic cells were … See more WebNov 29, 2024 · Cisplatin and pemetrexed response were quantified by proliferation and colony formation assay. Results Pemetrexed treatment of parental A549 cells increased mitochondrial mass over time. FACS-sorted paraclonal Mito-High cells featured increased mitochondrial mass and mitochondrial DNA copy number compared to the Mito-Low cells.
WebFeb 15, 2024 · We found that cisplatin response in model HNSCC cancer cells was modified by coculture with CAFs, was CAF-specific, and different patient-derived CAFs had a different "sensitizing ratio". Increased expression of VEGFA, PGE2S, COX2, EGFR, and NANOG in cancer cells was characteristic for the increase of resistance.
WebApr 2, 2024 · The assay was optimized using Analysis of Variance (ANOVA) to identify potential experimental confounders and Z-factor (Z), … optics yordan alvarez auto rated prospectWebTherapeutic efficacy of cisplatin-based treatment of late stage urothelial carcinoma (UC) is limited by chemoresistance. To elucidate underlying mechanisms and to develop new … portland maine dress shopsWebJan 18, 2024 · Cisplatin was administered by intraperitoneal injection in mouse. DNA damage, excision repair, and gene expression were assayed by Damage-seq, XR-seq, and RNA-seq. b PCA reveals tissue-specific... optics03Web(A,B) MTT assay. The cisplatin-resistant cells and their parental cells were exposed to different concentrations of cisplatin for 72 h and viability was related to untreated … optics.orgWebCisplatin is a platinum-based agent whose nephrotoxicity is thought related to the chloride in the cis position. Cisplatin gains entry into tubular cells via uptake by the OCT-2 … optics.org websiteWebAdjuvant cisplatin, 70 mg every three weeks, was given, for a total of 1680 mg. In February 1979, while he was receiving cisplatin, fever developed, accompanied by dark urine, a … optics yellowstoneWebUsing transporter-mediated cellular uptake assay, we identified wedelolactone (WEL), a medicinal plant-derived natural compound, is a competitive inhibitor of OCT2 and a noncompetitive inhibitor of MATE1. Wedelolactone showed a selectivity to inhibit OCT2 rather than MATE1. portland maine earthquake