Ded1p

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August undefined, 2024

WebJan 1, 2009 · The DEAD-box protein Ded1p/DDX3 is conserved from yeast to human. Various lines of genetic and biochemical evidence have indicated a role of the yeast Ded1p in translation and, most likely, in precursor mRNA splicing as well. In contrast, although recent studies have begun to reveal the function of the mammalian DDX3 in translation … WebAug 5, 2016 · To further examine the connection between Ded1p and eIF4A in the cell, we tested whether the eIF4A inhibitor hippuristanol exacerbated the temperature-sensitive …

Ded1p, a DEAD-box Protein Required for Translation …

WebThe Ded1 protein (Ded1p), a member of the DEAD-box family, has recently been shown to be essential for translation initiation in Saccharomyces cerevisiae. Here, we show that … WebMar 7, 1997 · The DED1 gene was originally identified as an essential open reading frame adjacent to HIS3 ( 6 ). Ded1p was hypothesized to function in nuclear pre-mRNA splicing … gumservis robert waszak

The current understanding of Ded1p/DDX3 homologs from …

WebMyDERP.org. The MyDERP.org account is a secure online resource where you can view and update your information, request an appointment with a membership services … WebDed1p leads to ATIS activation in 5′ UTRs, which decreases polysome formation on the main ORFs and thereby overall protein production. We conclude that Ded1p function suppresses the use of ATISs. WebDed1p formed a stable complex with RNA in the presence of ADP-BeF x, but stable complexeswerealsoformedwithADP-AlF 4 andADPPNP(Fig. 1D). Dissociation rate … gums cutting

The helicase Ded1p controls use of near-cognate …

A Co-Opted DEAD-Box RNA helicase enhances tombusvirus …

WebAug 20, 2015 · To probe whether Ded1p oligomerized in the absence of other proteins, we performed protein-protein crosslinking experiments with purified recombinant Ded1p in vitro (Figure 1 B).Multiple protomers crosslinked without RNA and the nonhydrolyzable ATP analog ADPNP, indicating formation of complexes with up to five Ded1p protomers … WebOct 26, 2007 · Dependence of Unwinding Rate Constants of Ded1p and Mss116p on the Length of the RNA Segment in the DuplexUnwinding rate constants for Ded1p with the blunt-end substrates were determined as in Figure 3 A, with 1000 nM Ded1p (left panel). Sequences for all substrates were identical; the numbers indicate the length of the RNA … bowling physicsWebDed1p associates with the translation pre-initiation complex at the mRNA entry channel and repressing the activity of Ded1p leads to the accumulation of RNA structure in 5' … bowling phrases

"WebThe conserved and essential DEAD-box RNA helicase Ded1p from yeast and its mammalian ortholog DDX3 are critical for translation initiation 1.Mutations in DDX3 are … " - Ded1p

Ded1p

WebAug 20, 2015 · Here we show that the DEAD-box helicase Ded1p oligomerizes in the cell and in vitro, and unwinds RNA as a trimer. Two protomers bind the single-stranded region of RNA substrates and load a third protomer to the duplex, which then separates the strands. ATP utilization differs between the strand-separating protomer and those bound to the … WebDed1p and show that it has RNA-dependent ATPase and ATP-dependent RNA helicase activities, both activities depending on the integrity of the DEAD motif. RNA binding studies also show that the affinity of Ded1p for RNA can be modulated by ATP and ADP, suggesting how RNA unwinding may be driven by ATP hydrolysis. EXPERIMENTAL PROCEDURES

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WebMay 11, 2024 · The Ded1p protein was genetically labeled with a green fluorescent dye. Credit: Technische Universitaet Dresden The conditions in the environment are subject to large fluctuations. In Germany, for ... Webdomain of Ded1p plays a major role in G4DNA binding, whereas the C-terminal domain is largely dispensable. The N-terminal domain of Ded1p contains RGG repeats, which have been shown in other proteins to bind quadruplex structures.18 The helicase domain is also likely involved in G4DNA binding because DN Ded1p retains some G4DNA binding activity.

WebOct 12, 2024 · Heat shock promotes the assembly of translation factors into condensates to facilitate the production of stress-protective proteins. How translation factors detect heat and assemble into condensates is not well understood. Here, we investigate heat-induced condensate assembly by the translation factor Ded1p from five different fungi, including … WebJan 1, 2009 · DExD/H-box RNA helicases are involved in almost all steps of the eukaryotic mRNA biogenesis. The DEAD-box protein Ded1p/DDX3 is conserved from yeast to …

WebJul 12, 2024 · The DDX3X/Ded1p subfamily has generated great interest because every member performs one or more nuclear functions and plays roles in the regulation of …

WebJan 24, 2024 · How Ded1p and its orthologs engage RNAs to impact translation initiation has been a longstanding, unresolved question. Here we show that Ded1p associates with the pre-initiation complex at the mRNA entry channel of the small ribosomal subunit and that the helicase unwinds mRNA structure ahead of the scanning pre-initiation complex. …

WebMore structured 5′UTRs may require additional helicases such as Ded1p in yeast , DHX29 in mammals , and others [46,47]. However, mRNA with ‘unstructured’ 5′UTRs can assemble SSU complexes at start codons without eIF4-group factors or ATP [16,42,45,48,49,50]. bowling photos freeWebDec 27, 2007 · Thus, Ded1p appears to be a bifunctional protein with roles in both translation initiation and P-body formation and thereby may play a role in controlling the … bowling photosWebMay 13, 1997 · So far, no other data concerning the function of Ded1p have been reported. Our results demonstrate that Ded1p is involved in translation initiation. DED1 is a multicopy suppressor of different cdc33 alleles and tif1–1. Furthermore, the ded1/spp81–3 allele is synthetically lethal with some translation initiation factor mutants. gum sensitive to cold waterWebMar 18, 2024 · Fig. 2 The N-terminal domain of Ded1p interacts with G4DNA. (a) Domain architecture of Ded1p. N-term and CTT contain intrinsically disordered regions. (b) A Ded1p variant lacking the C … gums feel itchyWebMay 14, 2024 · Ded1p is an ATP-dependent Asp-Glu-Ala-Asp (DEAD)-box RNA helicase. It resolves secondary structure in the 5′ untranslated regions (UTRs) of mRNAs to facilitate … bowling photos funnyWebDed1p’s nuclear role. First, Ded1p was identified in the purified spliceosome in yeast.14 Second, Ded1p is physically associated with spliceosomal U snRNPs15 and with pre-mRNA, splicing intermedi-ates and splicing products (Chang T-H, unpublished), consistent with a view that Ded1p initially interacts with spliceosome and bowling picture framesWebNov 24, 2024 · However, DDX3X separates shorter duplexes (≤ 16 bp) slower than Ded1p, and longer duplexes faster. Compared to Ded1p, DDX3X shows a more pronounced preference for substrates with unpaired regions 3′ to the duplex than to those with 5′ unpaired regions. Moreover, DDX3X is less potent than Ded1p in facilitating strand … bowling phrases funny