WebThe lentivirus-short hairpin RNA (shRNA) system is a widely used tool for RNA interference. Multiple factors may affect the RNA interference efficiency during lentivirus production … WebpLVX-shRNA2 价格: 1300元 联系方式: I47-825O-882O 相关技术服务: 质粒构建 基因合成 质粒大提 立刻购买 买家导航 载体基本信息 载体图谱质粒图谱和多克隆位点信息 载体简介 Description pLVX-shRNA2 is an HIV-1-based, lentiviral expression vector designed to express a small hair-pin RNA (shRNA) for RNA interference (RNAi) studies.
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WebThe Plasmids 101 eBook is designed to educate all levels of scientists and plasmid lovers and serves as an introduction to plasmids, allowing you to spend less time researching plasmid basic features and spend more time developing the clever experiments and innovative solutions necessary for advancing the field. 1. WebJul 1, 2010 · pLVX-Puro contains all of the viral processing elements necessary for the production of replication-incompetent lentivirus, as well as elements to improve viral titer, … parship.de login
Generation of an EFNB2-2A-mCherry reporter human embryonic ... - PubMed
WebDigest 1 ON -> purify -> Digest 2 -> purify (optional if buffer compatible with CIAP or any other) -> CIAP -> Inactivate or purify (I know u might lose a lot of DNA, but it works) Cite. 26th Jun ... WebJan 26, 2024 · For rescue experiment, the same short hairpin RNAs (shRNAs) were cloned into the pLVX-shRNA2-mCherry plasmid. The coding DNA sequence of FLOT1 was cloned into PCDH-MSCV-FLOT1-3 × FLAG-EF1-copGFP-T2A-puro plasmid to overexpress FLOT1, and then, synonymous mutations of CDS in the overexpression plasmid at FLOT1 shRNAs … WebMeSH Data Name Transduction, Genetic Concept Scope note: The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE. Tree numbers E05.393.350.800 Qualifiers parship belgique avis